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Writing in Nature in 1961, William Astbury described molecular biology as: "..so much a technique as an approach, an approach from the viewpoint of the so-called basic sciences with the leading idea of searching below the large-scale manifestations of classical biology for the corresponding molecular plan.It is concerned particularly with the forms of biological molecules and is predominantly three-dimensional and structural—which does not mean, however, that it is merely a refinement of morphology.It must at the same time inquire into genesis and function." Polymerase chain reaction is an extremely versatile technique for copying DNA.

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More recently, increased understanding of nuclease function has led to more direct DNA editing, using techniques such as zinc finger nucleases and CRISPR. The expressed nucleases then knock out and replace genes in the chromosome.

As of 2014 these approaches involve removing cells from patients, editing a chromosome and returning the transformed cells to patients.

In molecular biology, procedures and technologies are continually being developed and older technologies abandoned.

For example, before the advent of DNA gel electrophoresis (agarose or polyacrylamide), the size of DNA molecules was typically determined by rate sedimentation in sucrose gradients, a slow and labor-intensive technique requiring expensive instrumentation; prior to sucrose gradients, viscometry was used.

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